Cells in multicellular organisms possess a remarkable capacity to self-organize in ways that surpass other forms of active matter. These include coherent motility, self-healing, and branching phenomena that are controlled and timed in a programmed manner. The recent discovery of organoids, mini-organs that can be grown outside of the body, now allows one to study the underlying dynamics of these key processes directly for the first time. In this project you will use advanced confocal and light-sheet microscopy, as well as imaging analysis and mathematical modeling to track cells inside intestinal or lung organoid systems in 3D.
Fluorescent labeling will be used to follow cell movement and division in space and time, both on the cellular and sub-cellular level. The aim of this project is to uncover the fundamental mechanisms responsible for the spatial cell fate patterns and coordinated motility seen in these systems. These highly quantitative data will provide a unique insight into the dynamics that underlies complex 3D cellular organizations. This is a joint project between the Tans and van Zon labs at AMOLF Amsterdam and is in collaboration with the Clevers lab at the Hubrecht institute and HUB Organoid Technologies in Utrecht.
Biophysics, Quantitative Development Biology.
We are looking for an outstanding (bio)physicist, chemist, or biologist with skills in handling complex data and an interest in fundamental questions about development, and a strong drive to excel in a competitive international environment. Prior experience with quantitative (light-sheet) microscopy, organoid/tissue culture or quantitative analysis of single-cell behavior is not required, but is considered a plus. You will need to meet the requirements for an MSc-degree, to ensure eligibility for a Dutch PhD examination.
Conditions of employment
When fulfilling a PhD position at NWO-I, you will get the status of junior scientist. You will have an employee status and can participate in all the employee benefits NWO-I offers. You will get a contract for four years. Your salary will be up to a maximum of 2,937 euro gross per month. The salary is supplemented with a holiday allowance of 8 percent and an end-of-year bonus of 8.33 percent. You are supposed to have a thesis finished at the end of your four year term with NWO-I. A training programme is part of the agreement. You and your supervisor will make up a plan for the additional education and supervising that you specifically need. This plan also defines which teaching activities you will be responsible (up to a maximum of ten percent of your time). The conditions of employment of NWO-I are laid down in the Collective Labour Agreement for Research Centres (Cao-Onderzoekinstellingen), more exclusive information is available at this website under Personeelsinformatie (in Dutch) or under Personnel (in English). General information about working at NWO-I can be found in the English part of this website under Personnel. The 'Recruitment code' applies to this position.
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